Figure 7.

Structural changes upon reduction of ferric cyanide bound low-spin KpDyP. A, heme b, Asp-143, and cyanide (sticks) are represented from KpDyP subunit A (top) and subunit B (bottom) for the 5% reduced (blue, gray mesh, 6RPE) structure derived from the multicrystal approach and for the completely reduced routine single-crystal data set (purple, pink mesh, 6RPD). 2mF_o − DF_c electron density maps are represented for all modeled structural elements (contoured at 1.5 σ (cyanide alone) or 1 σ (rest)). B, flexibility of Asp-143 in subunit A (top) and subunit B (bottom) in the same colors as in A, representing the same states of reduction.