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. 2020 Aug 3;295(39):13640–13650. doi: 10.1074/jbc.RA119.011164

Figure 1.

Figure 1.

Expression of PGRN in human articular cartilage tissue in OA patients. a, safranin-O staining was performed on articular cartilage (n = 6/group). Mild OA indicates the articular cartilage of lateral femoral condyle from OA patients. Severe OA indicates the articular cartilage of medial femoral condyle from OA patients. Scale bar, 50 μm. b, immunohistochemistry and statistical analyses of PGRN expression in articular cartilage from normal humans and OA patients (n = 6/group). Scale bar, 50 μm. Brown-Forsythe and Welch ANOVA test with Dunnett's T3 multiple comparison test. c, Western blot analysis of expression of COL2A1, aggrecan, MMP13, ADAMTS5, and PGRN in articular cartilage, and the relative quantity was analyzed using ImageJ software. Unpaired two-tailed Student's t tests. d, mRNA levels of col2a1, aggrecan, MMP13, ADAMTS5, and PGRN were examined by real-time PCR in articular cartilage. Results were presented as gene expression levels in all groups normalized to controls. Unpaired two-tailed Student's t tests. Data were expressed as mean ± S.D. in each scatterplot. *, p < 0.05; **, p < 0.01; ***, p < 0.001.