Figure 3.

Effects of recombinant PGRN on SOX9 acetylation and nuclear translocation. a, Chondrocytes were treated with or without 100 ng/ml PGRN, and the level of acetylation of SOX9 was analyzed by IP–Western blot analysis. Unpaired two-tailed Student's t tests. b, chondrocytes transfected with control siRNA or SIRT1 siRNA cultured in the absence or presence of 100 ng/ml PGRN. The level of acetylation of SOX9 was analyzed by IP–Western blot analysis. One-way ANOVA and Tukey's multiple comparison test. c, subcellular localization of SOX9 was analyzed by Western blotting. d, immunofluorescence staining and statistical analyses of SOX9 nuclear translocation in chondrocytes. One-way ANOVA and Tukey's multiple comparison test. Scale bar, 50 μm. Data were expressed as mean ± S.D. in each scatterplot. *, p < 0.05; **, p < 0.01; ***, p < 0.001.