Figure 5.

Effects of recombinant PGRN on P65 acetylation and nuclear translocation. Chondrocytes were treated with or without 10 ng/ml TNFα, transfected with control siRNA or SIRT1 siRNA cultured in the absence or presence of 100 ng/ml PGRN. a, chondrocytes were lysed and the level of acetylation of P65 was analyzed by Western blotting. One-way ANOVA and Tukey's multiple comparison test. b, subcellular localization of P65 was analyzed by Western blotting. c and d, immunofluorescence staining and statistical analyses of P65 nuclear translocation in chondrocytes. Scale bar, 50 μm. One-way ANOVA and Tukey's multiple comparison test. e, ChIP analysis for recruitment of P65 to ADAMTS5 promoter in C28/I2 human chondrocyte cell line. One-way ANOVA and Tukey's multiple comparison test. Data were expressed as mean ± S.D. in each scatterplot. *, p < 0.05; **, p < 0.01; ***, p < 0.001.