Table 3.

Summary of thermal shift screening results^a

Screen	Compounds	Total hits	Positive hits	Negative hits
Primary (singleton)	30,013	1,129	492	637
Validation (triplicate)	1,129	282	176	106
HSQC	84	0

^a “Compounds” provides the total number of compounds tested in that screening step. “Positive hits” and “negative hits” list the number of molecules that shifted the Tm of HuPrP90-231 positively or negatively, respectively.