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. Author manuscript; available in PMC: 2020 Sep 28.
Published in final edited form as: Cell Rep. 2020 Aug 4;32(5):107995. doi: 10.1016/j.celrep.2020.107995

Figure 4. The Caspase-2-PIDDosome Contributes to the Switch in p53 Dynamics.

Figure 4.

(A) Western blot showing p53 and Mdm2 levels with or without ATM inhibitor (KU55933 10 μM) added 1 h prior to irradiation. Mdm2 levels represent p53 activity. Actin is shown as a loading control.

(B) Western blot showing p53 levels with or without ATM inhibition 16 h following irradiation. Chk2 phosphorylation is used to measure ATM activity. Actin is shown as a loading control.

(C) Schematic of the caspase-2-PIDDosome interaction with the p53-Mdm2 network following irradiation.

(D) p53 levels following CRISPR/Cas9 KO of PIDD1. Actin is shown as a loading control.

See also Figure S4.