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. Author manuscript; available in PMC: 2020 Sep 28.
Published in final edited form as: Cell Rep. 2020 Aug 4;32(5):107995. doi: 10.1016/j.celrep.2020.107995

Figure 5. Manipulating the Caspase-2-PIDDosome Alters Switching.

Figure 5.

(A) Heatmaps of scaled p53 intensity in the PIDD1 KO cell line (right panel) and control (left panel).

(B) Percent of switchers in control (n = 418 cells) and PIDD1 KO (n = 454 cells) populations as determined by k-means clustering.

(C) Percent of dividing cells in control (n = 418 cells) and PIDD1 KO populations (n = 454).

(D) Heatmaps of scaled p53 intensity following caspase-2 inhibition with 100 μM NH-23-C2 (right panel) or DMSO (control, left panel).

(E) Percent of switchers in control (n = 203 cells) and NH-23-C2 (n = 210 cells) populations as determined by k-means clustering.

(F) Percent of dividing cells in control (n = 203 cells) and NH-23-C2-treated (Casp2i) populations (n = 210).

(G) Heatmaps of scaled p53 intensities in PIDD1-overexpressing cells. Left panel is the uninduced control. In the right panel, PIDD1 was induced with doxycycline addition 24 h following irradiation (white dashed line).

(H) Percent of switchers in the control (n = 253 cells) and PIDD1-overexpressing cells (n = 153 cells) as determined by k-means clustering.

(I) Percent of cells that divided in the control (n = 253 cells) and PIDD1-overexpressing cells (n = 153 cells).

(B, C, E, F, H, I) Error bars represent standard error of the proportions.

See also Figure S4.