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Illustration of Several Steps of a BN-PAGE Experiment and Analysis
(A) Electrophoresis chamber running a 3%–12% BisTris gel at 4°C, after the removal of the dark blue cathode buffer and replacement with the light blue cathode buffer.
(B) Materials and equipment necessary for the assembling of the transfer sandwich for electroblotting.
(C) BN-PAGE gels used for in gel activity analysis of complex I (left), or complex IV (right), mounted in an acrylamide gel drying system.
