Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis

. 2020 Sep 3;1(2):100089. doi: 10.1016/j.xpro.2020.100089

Other Solutions

Name	Reagents
Anode Buffer	50 mL of 20× Native PAGE™ Running Buffer in 950 mL of deionized H₂O. Store at 4°C for up to 6 months
Dark Blue Cathode Buffer	50 mL of 20× Native PAGE™ Running Buffer and 50 mL of 20× Native PAGE™ Cathode Buffer Additive in 900 mL of deionized H₂O. Store at 4°C for up to 6 months
Light Blue Cathode Buffer	50 mL of 20× Native PAGE™ Running Buffer and 5 mL of 20× Native PAGE™ Cathode Buffer Additive in 945 mL of deionized H₂O. Store at 4°C for up to 6 months
Equilibration Buffer	15 mL of 10× eBlot L1 PVDF Membrane Equilibration buffer in 135 mL of deionized H₂O. Store at 4°C for up to 6 months
Coomassie Blue Dye Washing Buffer	62.5 mM Tris-HCl pH 6.8 and 2% SDS. Store at 15°C–25°C for up to 1 year