REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ATP5A	Abcam	Cat# ab14748 RRID: AB_301447
CORE2	Abcam	Cat# ab14745 RRID: AB_2213640
COX1	Abcam	Cat# ab14705 RRID: AB_2084810
COX5B	Santa Cruz	Cat# sc-374417 RRID: AB_10988066
NDUFB11	Abcam	Cat# ab183716 RRID: AB_2298378
SDHA	Abcam	Cat# ab14715 RRID: AB_301433
TOM20	SantaCruz	Cat# sc-11415 RRID: AB_2207533
β-ACTIN	Abcam	Cat# ab8227 RRID: AB_2305186
2˚ Ab-mouse	Rockland Immunochemicals	Cat# 610-103-121 RRID: AB_218457
2˚ Ab-rabbit	Rockland Immunochemicals	Cat# 611-1302 RRID: AB_219720
Cox1 (yeast)	Abcam	Cat# 110270
Sdh2 (yeast)	(Kim et al., 2012)	N/A
Atp2 (yeast)	(Liang and Ackerman, 1996)	N/A
Rip1 (yeast)	(Strogolova et al., 2012)	N/A
Porin (yeast)	Abcam	Cat# 110326
Chemicals, Peptides, and Recombinant Proteins
Dulbecco’s Modified Eagle Medium (DMEM)	Invitrogen	Cat# 11995
Fetal bovine serum (FBS)	Sigma	Cat# 12303C
Uridine	Sigma	Cat# U3003
Glutamax™	Gibco	Cat# 35050-061
Sodium Formate	Sigma	Cat# 71539
Trypsin-EDTA	Sigma	Cat# T4049
Bacto™ Yeast extract	BD	Cat# 212270
Bacto™ Peptone	BD	Cat# 211820
Galactose	Sunrise Science	Cat# 59-23-4
6-aminocaproic acid	Sigma	Cat# A2504
Bis-Tris	Sigma	Cat# B9754
Protease inhibitor cocktail	Sigma	Cat# P8340
Coomassie Brilliant Blue G	Bio-Rad	Cat# M2838
Apoferritin	Sigma	Cat# A3660
Lauryl maltose neopentyl glycol (LMNG)	Anatrace	Cat# NG310
n-dodecyl-β-δ-maltoside (DDM)	Sigma	Cat# 5172
Digitonin	Sigma	Cat# D141
Digitonin, High Purity	EMD Millipore	Cat# 300410
Isopropanol	Sigma	Cat# 24137
EDTA	Sigma	Cat# EX0550
Trizma® (Tris base)	Sigma	Cat# T1503
NaCl	Sigma	Cat# S3014
Triton X-100	Sigma	Cat# 9002-93-1
Methanol	Sigma	Cat# 646377
Glycine	Sigma	Cat# G7126
Iodonitrotetrazolium chloride	Sigma	Cat# 18377
NADH (Nicotinic acid dinucleotide sodium salt)	Sigma	Cat# N4256
Diaminobenzidine tetrahydrochloride (DAB)	Sigma	Cat# D5905
Cytochrome c	Sigma	Cat# C3131
Sucrose	Sigma	Cat# S8501
Critical Commercial Assays
SuperSignal™ West Femto Maximum Sensitivity Substrate	ThermoFisher	Cat# 34095
20× Native PAGE™ Running Buffer	Novex-Life Technologies	Cat# BN2001
20× Native PAGE™ Cathode Buffer Additive	Novex-Life Technologies	Cat# BN2002
eBlot L1 PVDF Membrane Transfer Buffer, 5×	Genscript	Cat# L00733
eBlot L1 PVDF Equilibration Buffer, 10×	Genscript	Cat# L00734
BCA Protein Assay kit	Sigma-Aldrich	Cat# BCA1-1KT
Gel Drying Solution, 1×	Bio-Rad	Cat# 1610752
Experimental Models: Cell Lines
HEK293T	ATCC	Cat# CRL-3216 RRID: CVCL_0063
Experimental Models: Saccharomyces cerevisiae strains
W303 I0	(Zambrano et al., 2007)	MATa, ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1, ρ+ I0
Other
Pre-cast Native PAGE 4%–16% Bis-Tris gels	Invitrogen	Cat# BN2012BX10
Pre-cast Native PAGE 3%–12% Bis-Tris gels	Invitrogen	Cat# BN2011BX10
eBlot™ L1 Fast Wet Transfer System for Mini Gels	Genscript	Cat# L00686
Immun-Blot® PVDF membrane	Bio-Rad	Cat# 1620177
BioTrace™ NT nitrocellulose	Pall Corporation	Cat# 66485
VWR® Pellet Mixer	VWR	Cat# 47747-370
TC20™ Automated Cell Counter	Bio-Rad	Cat# 1450102
Acrylamide Drying system	Invitrogen	Cat# NI2387
Scanner	HP	ENVY 5660
Teflon-glass homogenizer with smooth pestle	Thomas	3431D94 (170 mm) 3431E04 (88 mm)