Reagent | Final Concentration | Stock Concentration | Add to 4 L |
---|---|---|---|
Tris-HCl pH 8 | 10 mM | 1 M | 40 mL |
EDTA pH 8 | 1 mM | 0.5 M | 8 mL |
NaCl | 150 mM | n/a | 34.8 g |
Triton X-100 | 0.1% (v/v) | 10% (v/v) | 40 mL |
Deionized H2O | n/a | n/a | ~3.9 L |
Store at 15°C–25°C for up to 1 year.