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. 2020 Sep 3;1(2):100089. doi: 10.1016/j.xpro.2020.100089

Coomassie Staining Solution

Reagent Final Concentration Stock Concentration Add to 10 mL
Methanol 40% (v/v) 100% (v/v) 4 mL
Acetic acid 7% (v/v) 100% (v/v) 0.7 mL
Coomassie brilliant blue R250 0.25% (w/v) n/a 25 mg

Store at 15°C–25°C for up to 6 months.