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. 2020 May 16;1(5):100078. doi: 10.1016/j.xplc.2020.100078

Figure 3.

Figure 3

HY5 That is Restricted to the Hypocotyl Cannot Rescue the Lateral Roots Phenotypes of hy5 in Shade

(A and C) Representative fluorescence images and bright-field images of lateral root (LR) primordia (A) and LR tips (C) from seedlings expressing HY5 (green) under the control of different tissue-specific promoters or HY5p:HY5-GFP in the hy5 mutant background. As these constructs contained different fluorophores that were expressed at different levels, image capture settings were different for each image. Scale bars, A, 20 μm; C, 50 μm.

(B and D) LR density (the number of all emerged lateral roots divided by the total length of the main root) (B) and LR angle (D). In (D), the values of the angle between the longest lateral root tip and the main root are shown (see also Supplemental Figure 5). All images and measurements in (A)–(D) were taken of 10-day-old seedlings grown in white and far-red light (127 μE, R:FR = 0.6) LD at 21°C on media with sucrose. Different letters denote statistical differences (p < 0.05) among samples, as assessed by one-way ANOVA and Tukey HSD (n > 15).