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. 2020 Sep 28;15(9):e0230547. doi: 10.1371/journal.pone.0230547

Fig 6. Analysis of the polymorphism of the AT-rich microsatellite detected in the Cyclin-D-Binding Myb-Like Transcription Factor 1 gene (ms-DMTF1).

Fig 6

(A, B) Amplification of the ms-DMTF1 genomic sequence is restricted to the AEP strains, either unmodified (AEP1, AEP2) or transgenic (Q82-293, ecto-GFP, Wnt3::GFP, endo-GFP) lines. (C) Alignment of the ms-DMTF1 sequences. The color boxes indicate the AT-rich central region (salmon-pink) embedded within highly conserved regions (grey). Primer sequences used for amplification are indicated with black arrows. Numbers in brackets after the strain name indicate the number of independent positive sequencings, numbers at the 3’ end indicate the size of the PCR product and the number of AT-repeats (bold). Red writings indicate transcriptomic (t) or genomic (g) sequences available on HydrATLAS (HA) server [32, 40, 41], NHGRI web portal for the Hydra 2.0 genome (g2.0) [34] and Juliano transcriptomes (Jul) [38], or Compagen (Co) server [37, 42] (see S2 Table). (D) Graphical representation of the size of the ms-DMTF1 amplicons as deduced from sequencing data. Red color dots correspond to expected sizes, the grey dot indicates missing data.