Figure 10:

Overview of microfluidic device designs to mimic the gut. On the left of each panel, a schematic of the model is shown; and on the right, annotated representative microscopy images of the resulting model. (A) 2D monolayers on micro-molded scaffolds¹⁰⁶. (B) Microfluidic lumen interface device with integrated peristalsis. White arrows mark fluid flow while dark arrows indicate the motion of the stretchable surface¹¹². (C) Epithelial lumen interface on top of a microporous membrane cocultured with a square cross-sectioned endothelial lumen on the bottom. (D) Silk scaffolding tubular model with fibroblasts (blue), proliferative epithelial cells (green), and epithelial cells (red) ^{120, 122}. (E) Microfluidic ex-vivo gut co-culture with immune and brain cells from rats. Intact intestinal tissue is connected to input and output ports of the chamber (top), pumps controlling medium flow inside the lumen and in the external medium chamber¹¹⁸. (F) Channel network from the Organoplate platform containing cultured gut epithelial tube, ECM gel, and perfusion¹¹¹. (G) Gut tumor-on-a-chip gradient platform.¹²⁷ Reproduced with permission from Elsevier, from ref. 106 copyright 2018; from ref 120 copyright 2019; from ref 118, copyright 2017. Ref. 111 adapted with permission of Springer Nature, copyright 2017.