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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Chem Soc Rev. 2020 Sep 1;49(17):6402–6442. doi: 10.1039/d0cs00705f

Figure 5.

Figure 5.

A microfluidic bleeding device for studying vascular injury and hemostasis. (a) Image of microfluidic bleeding device. Microchannels are filled with dye for visualization. (b) The device consists of three PDMS layers: a vascular layer (VCL) containing a vascular channel and outlet channel, a valve layer (VL), and a valve actuator (VA) layer. (c) Endothelial cells are cultured in the vascular channel to confluency while the valve is closed. A wound is created by opening the valve layer via negative pressure in the valve actuator channel (pull) and by applied fluid pressure through the outlet channel (push). Subsequently, whole blood is perfused through the vascular channel which leads to the outlet channel. Scale bar is 50 μm. Adapted with permission from Sakurai et al., 201853.