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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Chem Soc Rev. 2020 Sep 1;49(17):6402–6442. doi: 10.1039/d0cs00705f

Figure 6.

Figure 6.

An organotypic blood vessel model for live-cell imaging of tumor invasion and intravasation. (A) Image of tubular HUVEC vessel formed in collagen type I gel. (B) Staining of CD31 (green) and nuclei (blue). (C) Scanning electron microscope image of collagen matrix. (D) Confocal image of vessel cross-section stained for CD31 (green) and nuclei (blue). Scale bar is 50 μm. (E) Confocal image of a vessel (green) co-cultured with HT1080 cells (red). Time-lapse images of (F) a single MDA-MB-231 cell (red) intravasating into a vessel, (G) a cluster of MDA-MB-231 cells (green) interacting with a vessel, and (H) angiogenic tip cell formation.63 Reproduced from ref. 63 with permission from the American Association for Cancer Research, copyright 2014.