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. 2020 Jul 15;31(15):1561–1569. doi: 10.1091/mbc.E20-02-0144

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FIGURE 2: — Knockdown of CB-enriched proteins disrupts miRNA biogenesis. JEG-3 (A, B) and HeLa (C–H) cells were transfected with siRNA for 120 h followed by RNA isolation and qRT-PCR to detect primary-miR-520 h (A), mature-miR-520 h (B), primary-let-7a (C, F), mature-let7a (D, G), and HMGA2 mRNA (E, H). The relative amount of a given amplicon in the control siRNA condition was set to 1. Data were derived from multiple biological (at least three) and technical (at least two) repeats, and error bars denote SD. ****p < 0.0001, ***p < 0.001, **p < 0.005.