FIGURE 3:

Altered processing of ectopic scaRNA9 on TDP-43 or SYNCRIP knockdown. (A) Schematic of full-length scaRNA9 annotating the mgU2-19 and mgU2-30 domains and other sequence elements (Enwerem et al., 2015; Poole et al., 2016, 2017). (B) Detection of the mgU2-30 fragment and full-length (FL) scaRNA9 by Northern blotting. RNA was isolated from cells transfected with siRNA then subsequently transfected with plasmid encoding scaRNA9. The bottom panel is a transformed section of the top panel containing the mgU2-30 signal. (C) Histogram generated from the quantification of the data shown in B and other experiments. For each condition, the mgU2-30 signal was divided by the FL scaRNA9 signal in that lane and normalized to that obtained with control siRNA. N = 3 biological repeats, *p < 0.05, error bars represent SD.