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. 2020 Jul 15;31(15):1570–1583. doi: 10.1091/mbc.E20-01-0067

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© 2020 Gerien et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Mso1 colocalizes and interacts with the SM protein Sec1. (A) Mso1 and Sec1 colocalize at the division site and at cell tips. (B) Mso1-mEGFP ectopically targeted to mitochondria by Tom20-GBP can recruit Sec1-tdTomato. (C) Mso1-mEGFP is able to pull down Sec1-13Myc from S. pombe cell lysates in co-IP assay. (D) SDS–PAGE gel showing in vitro binding assays between Sec1 and Mso1 using purified proteins. MBP-Sec1 can pull down full-length Mso1 and Mso1(1-70), but not Mso1(71-151). Bead bound MBP-Sec1 or an MBP control was incubated with Mso1, Mso1(1-70), or Mso1(71-151). (E) Sec1 localization in mso1∆ cells grown at 25°C or 36°C for 2 h. (F) Sec1-tdTomato intensity at the division site at 25°C. WT, n = 27 cells; mso1Δ, n = 29 cells. Bars, 5 µm.