Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 15;31(15):1584–1594. doi: 10.1091/mbc.E19-10-0578

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Fox et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 5: — Prostaglandins regulate Fascin to promote border cell migration and control cluster morphology. (A, B) Maximum projections of two to four confocal slices of S9 follicles of the indicated genotypes; anterior is to the left. (A) fascin^sn28/+; pxt^EY/+. (B) fascin^sn28/+; pxt^f/+. Merged images: somatic cell stain (Hts and FasIII), green; DAPI (DNA), blue; and phalloidin (F-actin), red. Orange dashed lines indicate the position of the outer follicle cells. (C) Graph of the migration index quantification during S9 for the indicated genotypes. Dotted line at 1 indicates an on-time migration. Each circle represents a single border cell cluster; n = number of follicles. Each line indicates the average and the whiskers indicate the SD. ****, p < 0.0001; **, p < 0.01 (one-way ANOVA with Tukey’s multiple comparison test). (D, E) Maximum projections of two to four confocal slices of S9 follicles of the indicated genotypes stained with the somatic cell stain (Hts and FasIII, white). (D) fascin^sn28/+; pxt^EY/+. (E) fascin^sn28/+; pxt^f/+. Yellow arrowheads denote tails attached to the cluster. (F) Graph of the quantification of border cluster length from follicles of the indicated genotypes. Each circle represents a single border cell cluster; n = number of follicles. Each line indicates the average and the whiskers indicate the SD. ****, p < 0.0001; **, p < 0.01; and *, p < 0.05 (one-way ANOVA with Tukey’s multiple comparison test). While heterozygosity for mutations in pxt or fascin do not affect border cell migration or cluster morphology, double heterozygotes (fascin−/+; pxt−/+) exhibit delayed border cell migration (A–C) and elongated clusters (D–F). Scale bars = 50 μm.