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. 2020 Jul 15;31(15):1611–1622. doi: 10.1091/mbc.E19-07-0406

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© 2020 Hennessey et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Nek8445 is required for cytokinesis. (A) Multiplex immunoblot showing Nek8445-HA depletion 12 and 24 h after Nek8445 antisense and control morpholino treatment. (B) Representative still images from a 4D time-lapse movie showing failed cytokinesis in Nek8445-depleted cells (see Supplemental Video S1). T = 0 set to the initiation of cytokinesis, which occurs ∼7 min after the initiation of mitosis as indicated by anterior flagella reorganization. (C) Histogram of cytokinesis timing. Only 13% of Nek8445-depleted cells complete cytokinesis within 2 min vs. 90% for the control treatment (n = 88 control morpholino, n = 56 Nek8445 KD). Scale bar = 5 µm.