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. 2020 Jul 15;31(15):1611–1622. doi: 10.1091/mbc.E19-07-0406

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© 2020 Hennessey et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — Nek8445 relocalizes during mitosis to associate with axoneme microtubules. (A) Still images from a time-lapse movie of Nek8445-mNG showing Nek8445 translocating from the cell cortex to the nuclear envelope, and axoneme microtubules (see Supplemental Video S2). White arrowheads indicate Nek8445 in association with cytoplasmic axonemes; cyan arrowhead marks nascent axonemes pointing into the developing furrow. (B) Immunofluorescence analysis of Nek8445-HA (red), microtubules (6-11B-1 antibody; green), and nuclei (DAPI; blue) throughout the cell cycle. Scale bar = 5 µm.