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SEM analysis reveals Nek8445-depleted cells have caudal axoneme exit defects. Control morpholino and Nek8445 knockdown cells processed for SEM 12 h after morpholino treatment. Note the shorter cell length and absence of caudal flagella (cf) at the anterior apex of the cell. White arrowhead indicates an abnormal membrane protrusion associated with a caudal flagellum that eventually exits the cell body at an abnormal position. Scale bar = 5 µm.
