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. 2020 Jul 15;31(15):1611–1622. doi: 10.1091/mbc.E19-07-0406

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© 2020 Hennessey et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 8: — Structured Illumination superresolution imaging of δ-giardin-3HA and tubulin (6-11B-1 antibody) in morpholino-treated cells. Nek8445-depleted cells lack median bodies, a funis, and the axonemes have a fraying defect. Control inset shows a magnified view of funis microtubules. Middle inset shows that the Nek8445-depleted cells lack both a median body and funis. The bottom image shows an example of a Nek8445-depleted cell with abnormal disk morphology and splayed axoneme tips. Scale bar = 5 µm.