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. 2020 May 20;10(10):e3630. doi: 10.21769/BioProtoc.3630

Figure 1. Bronchoalveolar lavage harvest using 20 gauge x 1-inch Introcan Safety IV catheter.

Figure 1.

A. Pin mouse in a supine position on top of Styrofoam. B. Cut skin to reveal the thoracic and peritoneal areas. C. Cut through the peritoneal wall and then cut diaphragm to expose lung cavity. D. Cut through submandibular salivary glands and sternothyroid muscle to expose trachea (Rib cage removed for photo clarity). E. Make a small incision in the center of the trachea using microscissors (white arrow). F. Thread catheter into trachea. G-H. Observe as lung expands upon injection of 1 ml PBS.