FIGURE 6:

U2OS mechanics are only modestly affected by 1D microline width. (A) Schematic representation of an AFM cantilever engaging a cell seeded on 1D microlines of fibronectin. (B) MLCT-Bio cantilever (lever C) indenting living cells. (C) Young’s modulus values of U2OS cells seeded on microlines with widths ranging from 5 to 100 µm. Each bar represents the median value of all the Young’s modulus values collected on all the cells for each category, plotted with interquartile range as error bars. Statistical significance was determined using the Mann-Whitney test between each pair of categories (***, p < 0.0001; n = 2048, 2560, 2048, 2816, 3072, and 3072 force curves on 8, 10, 8, 11, 12, and 12 cells across six independent experiments. For each cell, a 16 × 16 force map was recorded on the supranuclear region of the cell body).