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. 2020 Jul 21;31(16):1654–1662. doi: 10.1091/mbc.E20-02-0097

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© 2020 Todorovski et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Migration tracking of MCF10A, U2OS, 143B, and MDA-MB-231 cells on 3 kPa vs. 40 kPa hydrogels showed increased migratory trends on stiff substrates. (A) Rose plots corrected to 0,0 showing representative tracks taken by cells cultured on 3 kPa and 40 kPa (n = 20). (B) Total distance traveled confirmed that cells from all cell lines cultured on 40 kPa hydrogels migrated a greater distance (n = 80) compared with cells cultured on 3 kPa hydrogels (n = 80). Data are shown as mean ± SEM. ****, p < 0.0001.