Table 1.
DNA name | Sequences and modifications (from 5′ to 3′) |
---|---|
E gene SNA-DNAa | A*A*A*A*A*A*A*A*AAAA GTTACACTAGCCATCCTTAC |
E gene linker probeb | GCGCAGTAAGGATGGCTAGTGTAAC |
Forward primer | ACAGGTACGTTAATAGTTAATAGCGT |
Reverse primer | ATATTGCAGCAGTACGCACACA |
E gene real-time probec | FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ |
Phosphorothioate modification is denoted by “*”.
The underlined bases represent the palindromic sequence.
FAM: 6-carboxyfluorescein; BBQ: blackberry quencher.