Table 2.
Dilution Factor | Resultsa |
|||||
---|---|---|---|---|---|---|
Real time RT-PCR (Ct value) |
Past-PCR colorimetric method |
|||||
No. 1 | No. 2 | No. 3 | No. 1 | No. 2 | No. 3 | |
1 | 21.24 | 23.76 | 23.98 | + | + | + |
10 | 23.96 | 26.44 | 27.33 | + | + | + |
100 | 26.85 | 30.06 | 31.17 | + | + | + |
1000 | 28.31 | 33.07 | 33.97 | + | + | + |
10,000 | 33.12 | 36.86 | 37.60 | + | + | + |
100,000 | 37.21 | NAb | NA | + | + | + |
Positive and negative results are indicated by "+" and "−".
NA, Not Applicable.