| Strain, strain background (M. musculus, Tie2-Cre, C57BL6/J background) |
B6.Cg-Tg(Tek-cre) 1Ywa/J |
Jackson Laboratories |
RRID:IMSR_JAX:008863
|
|
| Strain, strain background (M. musculus, smMHC-Cre/eGFP, C57BL6/J background) |
B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J Mus musculus
|
Jackson Laboratories |
RRID:IMSR_JAX:007742
|
|
| Strain, strain background (aSM22-Cre, C57BL6/J background) |
B6.Cg-Tg(Tagln-cre)1Her/J Mus musculus
|
Jackson Laboratories |
RRID:IMSR_JAX:017491
|
|
| Strain, strain background (TdTomato reporter Ai14, C57BL6/J background) |
B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus
|
Jackson Laboratories |
RRID:IMSR_JAX:007909
|
|
| Strain, strain background (P2ry floxed, C57BL6/J background) |
P2ry2f/f
Mus musculus
|
PMID:27856454
|
|
Gifted by Dr. Cheike Seye (Indiana Univ.) |
| Transfected construct (M. musculus) |
AAV2-Myh11p-eGFP-2A-mP2ry2 |
This paper |
|
Custom AAV product from Vector Biolabs |
| Transfected construct (M. musculus) |
AAV2-Myh11p-eGFP |
This paper |
|
Custom AAV product from Vector Biolabs |
| Genetic reagent (TaKaRa TaqTM DNA Polymerase) |
Taq polymerase |
Promega |
R001A |
|
| Antibody |
(mouse monoclonal) anti-α smooth muscle actin |
Sigma |
A5228 |
(1:100 dilution) |
| Sequence-based reagent |
Taqman probe Gapdh
|
ThermoFisher |
Mm99999915_g1 |
|
| Sequence-based reagent |
Taqman probe Rbfox3
|
ThermoFisher |
Mm01248771_m1 |
|
| Sequence-based reagent |
Taqman probe Aldh1l1
|
ThermoFisher |
Mm03048957_m1 |
|
| Sequence-based reagent |
Taqman probe Acta2
|
ThermoFisher |
Mm00725412_s1 |
|
| Sequence-based reagent |
Taqman probe Flt1
|
ThermoFisher |
Mm00438980_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx1
|
ThermoFisher |
Mm00435460_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx2
|
ThermoFisher |
Mm00462952_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx3
|
ThermoFisher |
Mm00523699_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx4
|
ThermoFisher |
Mm00501787_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx5
|
ThermoFisher |
Mm00473677_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx6
|
ThermoFisher |
Mm00440591_m1 |
|
| Sequence-based reagent |
Taqman probe P2rx7
|
ThermoFisher |
Mm01199500_m1 |
|
| Sequence-based reagent |
Taqman probe P2ry1
|
ThermoFisher |
Mm02619947_s1 |
|
| Sequence-based reagent |
Taqman probe P2ry2
|
ThermoFisher |
Mm02619978_s1 |
|
| Sequence-based reagent |
Taqman probe P2ry4
|
ThermoFisher |
Mm00445136_s1 |
|
| Sequence-based reagent |
Taqman probe P2ry6
|
ThermoFisher |
Mm02620937_s1 |
|
| Sequence-based reagent |
Taqman probe P2ry12
|
ThermoFisher |
Mm01950543_s1 |
|
| Sequence-based reagent |
Taqman probe P2ry13
|
ThermoFisher |
Mm01950543_s1 |
|
| Sequence-based reagent |
Taqman probe P2ry14
|
ThermoFisher |
Mm01952477_s1 |
|
| Chemical compound, drug |
Papain |
Sigma |
P4762 |
|
| Chemical compound, drug |
1,4-Dithioerythritol |
Sigma |
D8255 |
|
| Chemical compound, drug |
Collagenase IV |
ThermoFisher |
1714019 |
|
| Chemical compound, drug |
Bovine Serum Albumin |
Sigma |
A2153 |
|
| Chemical compound, drug |
Trypsin Inhibitor |
Sigma |
T9253 |
|
| Chemical compound, drug |
U46619 |
Tocris |
1932 |
|
| Chemical compound, drug |
Trans-ACPD |
Tocris |
0187 |
|
| Chemical compound, drug |
AR-C 118925XX |
Tocris |
4890 |
|
| Chemical compound, drug |
PSB 1114 |
Tocris |
4333 |
|
| Chemical compound, drug |
POM 1 |
Tocris |
2689 |
|
| Chemical compound, drug |
1,3-dimethyl-8-phenyl-xantine (8-PT) |
Sigma |
P2278 |
|
| Chemical compound, drug |
Prostaglandin E2 |
Cayman Chemical |
14010 |
|
| Chemical compound, drug |
L-798, 106 |
Cayman Chemical |
11129 |
|
| Chemical compound, drug |
Papaverine |
Sigma |
P3510 |
|
| Chemical compound, drug |
Diltiazem |
Tocris |
0685 |
|
| Commercial assay, kit |
Taqman Gene Expression Cells-to-CT kit |
ThermoFisher |
AM1728 |
|
| Commercial assay, kit |
Taqman Fast Advanced Master Mix |
ThermoFisher |
4444557 |
|
| Software, algorithm |
ImageJ |
NIH |
RRID:SCR_003070
|
Version 2.0.0 |
| Software, algorithm |
Macro (for vessel analysis) |
PMID:28387198
|
|
|
| Software, algorithm |
Ponemah |
DSI |
RRID:SCR_017107
|
Version 5.23 |
| Software, algorithm |
QuantStudio Design and Analysis |
ThermoFisher |
RRID:SCR_018712
|
Version 1.5.1 |
| Software, algorithm |
Prism 7 |
GraphPad |
RRID:SCR_011323
|
Version 7.03 |
| Other |
(Griffonia Simplicifolia Lectin I) isolectin B4, Dylight 594 |
Vector Laboratories |
SL-1207-.5 |
(6 µg/mL dilution) |
