Table 2. Cardiorespiratory and metabolic parameters under room air conditions in control and P2Y2 cKO mice.
| A | TaglnCre or P2ry2f/f only | ||
|---|---|---|---|
| Frequency (breaths/minute) | Tidal volume (mL/g x 10−3) |
Minute ventilation (mL/min/g) | |
| Room air | 163.4 ± 7.1 | 6.8 ± 0.4 | 1.1 ± 0.1 |
| 0% CO2/100% O2 | 149.1 ± 5.7 | 6.7 ± 0.5 | 1.0 ± 0.1 |
| 3% CO2/97% O2 | 236.6 ± 8.5 **** | 8.7 ± 0.4 **** | 2.1 ± 0.1 **** |
| 5% CO2/95% O2 | 306.0 ± 9.4 **** | 12.9 ± 0.6 **** | 4.0 ± 0.3 **** |
| 7% CO2/93% O2 | 351.6 ± 9.8 **** | 17.1 ± 1.4 **** | 6.0 ± 0.3 **** |
| B | P2Y2 cKO | ||
| Frequency (breaths/minute) | Tidal volume (mL/g x 10−3) |
Minute ventilation (mL/min/g) | |
| Room air | 149.8 ± 7.2 | 6.7 ± 0.5 | 1.0 ± 0.1 |
| 0% CO2/100% O2 | 130.1 ± 7.4 | 7.1 ± 0.3 | 0.9 ± 0.1 |
| 3% CO2/97% O2 | 213.7 ± 6.5 **** | 8.0 ± 0.3 | 1.7 ± 0.1 ** |
| 5% CO2/95% O2 | 256.2 ± 9.6 ### **** | 10.4 ± 0.4 #### **** | 2.7 ± 0.2 #### **** |
| 7% CO2/93% O2 | 291.3 ± 7.5 #### **** | 13.3 ± 0.5 #### **** | 3.9 ± 0.1 #### **** |
| C | P2Y2 cKO rescue | ||
| Frequency (breaths/minute) | Tidal volume (mL/g x 10−3) |
Minute ventilation (mL/min/g) | |
| Room air | 156.3 ± 9.0 | 5.6 ± 0.1 | 0.9 ± 0.2 |
| 0% CO2/100% O2 | 158.3 ± 12.8 | 6.8 ± 0.1 | 1.1 ± 0.3 |
| 3% CO2/97% O2 | 254.0 ± 6.2 ## **** | 9.5 ± 0.1 *** | 2.4 ± 0.3 **** |
| 5% CO2/95% O2 | 305.1 ± 9.8 #### **** | 14.0 ± 0.1 ## **** | 4.3 ± 0.4 #### **** |
| 7% CO2/93% O2 | 335.6 ± 4.1 ### **** | 17.0 ± 0.2 ### **** | 5.7 ± 0.6 #### **** |
*,difference from 0% CO2; #, differences between genotypes under an experimental condition (two-way ANOVA with Tukey's multiple comparison test). Two symbols = p < 0.01, three symbols = p < 0.001, four symbols = p < 0.0001.