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. Author manuscript; available in PMC: 2021 Jun 19.
Published in final edited form as: ACS Chem Biol. 2020 May 11;15(6):1358–1369. doi: 10.1021/acschembio.9b01027

Figure 3. Cytosolic delivery of antisense SF3B1 PNAs reduces SF3B1 protein production.

Figure 3.

(A) Illustrations of the sense and antisense LP-SF3B1 sequences. (B) Cartoon illustration of the SF3B1 mRNA transcript with the translated and untranslated regions drawn according to scale for their respective lengths. (C) Immunoblot showing the suppression of the SF3B1 protein in CRISPRneutral and CRISPRcopy loss cell lysate after incubation with 250 nM sense or antisense LP-SF3B1 and 50 nM PA. (D) Immunoblot showing dose-dependent suppression of the SF3B1 protein in CRISPRneutral cell lysate after incubation with 8, 40, or 250 nM sense or antisense LP-SF3B1 and 50 nM PA. Cell lysates were collected after a four-day incubation time.