Fig. 1. Effects of JH4 pretreatment on the H₂O₂-induced cell death of ASCs. (A) H₂O₂-induced cell death of ASCs. ASCs were treated with 0.5 mM H₂O₂ or vehicles for 48 hours, and cell images were taken under microscope. (B) Dose-dependent effects of H₂O₂ on cell viability of ASCs. ASCs were treated with the indicated concentrations of H₂O₂ for 48 hours, and cell viability was measured using the MTT assay. (C) Effects of JH4 on H₂O₂-induced cell death of ASCs. ASCs were pretreated with the indicated concentrations of JH4, treated with 0.5 mM H₂O₂ for 48 hours, followed by measurement of cell viability. (D) Effects of JH4 on H₂O₂-induced morphological change of ASCs. ASCs were treated with 0.5 mM H₂O₂ for 48 hours in the absence or presence of 1 μM JH4, followed by capturing cell images. (E) Effects of JH4 on cell viability of ASCs. ASCs were treated with the indicated concentrations of JH4 for 48 hours, followed by measurement of cell viability. The data represent the mean ± standard error of the mean. Scale bar, 200 μm.

H₂O₂, hydrogen peroxide; NS, not significant; ASC, adipose tissue-derived mesenchymal stem cell; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

^*p<0.001 vs. control; ^†p<0.05; ^‡p<0.01; ^§p<0.001 vs. H₂O₂ only by 1-way analysis of variance.