Fig. 3. Effects of JH4 on ASC-stimulated blood perfusion and ischemic limb salvage. The ischemic limbs were injected with ASCs in the absence or presence of JH4. For negative control, HBSS was injected into ischemic limbs. (A) Representative images of the ischemia-induced mouse hind limb on day 28. (B) Quantitative analysis of blood flow measured by laser doppler perfusion imaging analysis. (C) Analysis of the necrosis score on day 28. (B, C) The data represent the mean ± standard error of the mean.

HBSS, Hank's balanced salt solution; ASC, adipose tissue-derived mesenchymal stem cell; ANOVA, analysis of variance.

^*p<0.05 vs. HBSS; ^†p<0.05 vs. ASCs by 2-way ANOVA; ^‡p<0.05; ^§p<0.001 vs. HBSS by 2-way ANOVA (n=6).