Fig. 1. Analysis of RNA sequencing data from MI and ischemic stroke (MCAO) models. (A) Changes in the expression of marker genes from each experimental model. The mRNA level change of Timp1 was used to determine the reliability of the MI model. For the MCAO model, the mRNA level of the Gfap gene, which encodes glial fibrillary acidic protein, was used as a marker. The p-value was calculated using Student's t-test and described as asterisks. (B) The analysis scheme to identify altered lncRNAs common to MI and MCAO models. Details of the analysis procedure are described in the materials and methods section.

Timp1, tissue inhibitor of metalloproteinase 1; Gfap, glial fibrillary acidic protein; MI, myocardial infarction; MCAO, middle cerebral artery occlusion; lncRNA, long non-coding RNA; mRNA, messenger RNA.

^*p<0.01, ^†p<0.001, ^‡p<0.0001.