Fig. 3. Hsa_circ_0005556 targets miR-4270 as a miRNA sponge. (A) Relative miRNA expression was measured after the transfection of MGC-803 cells. (B) Schematic representation of the binding sites for hsa_circ_0005556 and miR-4270. (C) The luciferase reporter assay was performed to evaluate the interaction between miR-4270 and hsa_circ_0005556. (D) The expression of miR-4270 in gastric cancer and para-carcinoma tissues was measured using qRT-PCR. (E) The expression of miR-4270 was measured using qRT-PCR in different groups. (F) The proliferation of MGC-803 and MKN-28 cells was tested by CCK-8 assay in different groups. (G) Cell invasion was measured in MGC-803 and MKN-28 cells after transfection (100×).

qRT-PCR = quantitative real-time polymerase chain reaction; CCK-8 = Cell Counting Kit-8; NC = negative control.

^*P<0.05 and ^†P<0.01.