Skip to main content
. 2020 Sep 23;147(18):dev193094. doi: 10.1242/dev.193094

Fig. 1.

Fig. 1.

Doppel is expressed in vascular endothelial cells of angiogenic blood vessels in the developing brain. (A) Analysis of the brain RNA-seq database reveals that Prnd mRNA is expressed exclusively in brain endothelial cells isolated from Tie2-GFP mice at P7. The expression data were graphed as fragments per kb of transcript per million (FKPM). (B) Analysis of the genepaint.org database reveals Prnd mRNA expression in blood vessels of the E14.5 embryonic mouse brain (black arrows). (C) Quantitation of Prnp and Prnd mRNA levels in the total mouse brain at different postnatal ages using qRT-PCR. Note that Prnd mRNA levels in the neonatal brain are reduced after P7 and are very low in the adult mouse brain. Prnd mRNA is absent in the adult Prnd−/− mouse brain. Differences in Prnp mRNA levels between wild-type and Prnd−/− adult brain samples are not statistically significant. (D) CD31+ vascular endothelial cells were fractionated from Prnd wild-type (+/+) or Prnd knockout (−/−) neonatal mouse brains (P7) and doppel protein expression was determined by immunoblotting. Note the absence of doppel expression in the Prnd−/− brain endothelial cell fractions. (E) Sagittal sections from wild-type brain tissue from different embryonic (14.5 and E17.5) and postnatal (P7 and P21) ages were immunohistochemically labeled with anti-doppel antibodies. Note that doppel is expressed in vascular endothelial cells of the embryonic and neonatal brains (black arrows) but is reduced in the more mature P21 brain (red arrows). Scale bars: 50 µm.