Figure 6.

BOK Is Differentially Expressed in Human and Mouse AD Brains

(A) Single-nuclei RNA-seq generated from the entorhinal cortex of human AD and control postmortem brains (data from Grubman et al., 2019). UMAP manifold of single nuclei colored by BOK's normalized expression (red means higher expression level) together with genotype and cell type maps.

(B) Single-nuclei RNA-seq generated from the parietal lobe of human AD. t-SNE manifold of single nuclei colored by BOK (normalized expression) together with cell types. Data from Del-Aguila et al. (2019).

(C) UMAP manifold of factor activities colored by Bok's normalized expression. The manifold is the same as in Figure 2C. The regions corresponding to the thalamus and CA3 are labeled. Localization of Bok denoted with red dots.

(D) Alteration of BOK protein level and localization in AD hippocampi. Representative images of coronal CA3 sections from human control (Ctrl) and Alzheimer (AD, Braak VI) hippocampi. Scale bars, 20 μm.

(E) Quantification of images of BOK staining in hippocampi. The cellular and nuclear BOK fluorescence intensities were quantified within CA2/CA3 cells from Ctrl and AD hippocampi (cellular Ctrl, n = 237; AD, n = 493) (nuclear Ctrl, n = 86; AD, n = 115). Data are presented as mean ± SEM. Statistical significance was determined using per brain mean value; three brains per category were analyzed.

(F) Alteration of BOK protein levels and localization in AD thalami. Representative images of coronal sections from human control (Ctrl) and Alzheimer (AD, Braak VI) thalami. Scale bars, 20 μm.

(G) Quantification of images of BOK staining in thalami. The cellular and nuclear BOK fluorescence intensities were quantified within cells from Ctrl and AD thalami (cellular Ctrl, n = 127; AD, n = 133) (nuclear Ctrl, n = 74; AD, n = 67). Data are presented as mean ± SEM. Statistical significance was determined using per brain mean value; three brains per category were quantified.