Figure 1.

SOD2 Is Essential for the Expression of Neuroectodermal Genes

(A and B) mRNA (A) and protein (B) levels of SOD2 in endoderm, mesoderm, and neural differentiation of mESCs. mRNA levels are plotted as mean ± SE of biological triplicates and statistical significance has been calculated using paired Student's t test, ∗p < 0.05, ∗∗p < 0.01. (C) mRNA expression of SOD2 and specific markers in neural (i) and oligodendrocyte (ii) differentiation of mESCs. Comparison of mRNA (D) and protein (E) levels of neural markers in SOD2 over-expression against the vector control. (F) Comparison of mRNA levels of neural markers in the vector control, with and without BMP4 treatment, and SOD2 over-expression with BMP4 treatment. (G) Transcript analysis of germ layer markers in endodermal (i), mesodermal (ii), and ectodermal (iii) differentiation from mESCs upon SOD2 knockdown. Expression analysis of early neural marker SOX1 upon SOD2 knockdown in early neural differentiation of mESCs by immunoblotting (H) and immunofluorescence (I). Scale bar represents 50 μm. Un-cropped full western blot images are available in Data S1. List of primers used for transcript analysis and antibodies used for protein detection are available in Tables S1 and S2, respectively.