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. 2020 Sep 15;23(10):101564. doi: 10.1016/j.isci.2020.101564

Figure 2.

Figure 2

SOD2 Specifically Modulates Early Neural Differentiation

(A) Modulation of protein expression of SOD2 across neural differentiation of mESCs. (B) Analysis of ROS levels using DCFHDA staining during early and late neural differentiation of mESCs. (C) mESCs were transfected with inducible SOD2 over-expression construct and 48 h after addition of doxycycline, cells were harvested and expression of SOD2 analyzed by western blotting. (D) Gene expression analysis of specific neural markers upon SOD2 over-expression induced during (i) early neural differentiation (days 0–3) and (ii) late neural differentiation (days 3–7). (E) mESCs were transfected with inducible SOD2 shRNA construct and 48 h after addition of doxycycline, cells were harvested and expression of SOD2 analyzed by western blotting. (F) Transcript analysis of specific neural markers upon SOD2 knockdown induced during (i) early neural differentiation (days 0–3) and (ii) late neural differentiation (days 3–7). Un-cropped full western blot images are available in data S1. List of primers used for transcript analysis and antibodies used for protein detection are available in Tables S1 and S2, respectively.