Figure 4.

iNPLCs Differentiate to Mature Neurons In Vitro and In Vivo

(A and B) mRNA levels of matured neural markers (A) and glial markers (B) in iNPLC clones differentiated in vitro. (C) Representative immunofluorescence image of matured neuronal and glial markers in iNPLC clones differentiated in vitro. (D) Representative immunohistochemistry image showing in vivo maturation of injected iNPLC cells harboring GFP plasmid. Mean ± SE, n = 3 independent experiments. Scale bar represents 50 μm. List of primers used for transcript analysis and antibodies used for protein detection are available in Tables S1 and S2, respectively.