Figure 5.

SOD2 Enhances Mitochondrial Fusion Process

(A) Representative confocal images showing modulation in mitochondrial architecture upon SOD2 knockdown in P40H1 cell line. The enlarged image of the boxed region is represented beside each main image. (B) Quantification of mitochondrial length upon SOD2 knockdown in P40H1 cell line. (C) Representative confocal images showing modulation in mitochondrial architecture upon over-expression of wild-type and redox activity mutant constructs of SOD2 in P40H1 cell line. (D) Change in mitochondrial length upon the over-expression of WT and mutant SOD2 in P40H1 cell line. (E) Mitochondrial superoxide levels upon over-expression of SOD2 wild-type and mutant SOD2 constructs as quantified using Mitosox Red staining. (F) Quantification of percentage of mitochondrial contacts upon SOD2 over-expression. (G) (i) Schematic representation depicting the bio-complementation assays used to quantify the mitochondrial fusion process and (ii) the relative fold change in mitochondrial fusion upon SOD2 over-expression as assayed using bio-complementation assay. Data representative of mean ± SE, n = 3 independent experiments, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bar represents 100 pixels. Twelve images per condition were analyzed and length of ∼400 mitochondria was calculated. See also Videos S1 and S2.