Figure 6.

Protein-Protein Interaction (PPI) and a Network Model for Leptin Action in Puberty Onset via the PMv

(A) Nodes in first connection with Lepr are genes predicted to be direct targets of leptin. Nodes in second connection are DEGs common in MBHp and PP versus Di RNA-seq, experimentally predicted to be downstream targets of leptin. Nodes in third connection (green) represent PP versus Di DEGs experimentally determined to interact with leptin targets. Purple, DEGs; orange, rDEGs; solid lines, interactions identified by STRING; dotted lines, predicted interactions.

(B) Fluorescent images showing partial colocalization of LepRb eGFP and Laminin (Lam) immunoreactivity (-ir). Arrows indicate dual-labeled neurons. Scale bar: 200 μm.

(C) Quantification of dual-labeled neurons in the PMv of WT, Lep^ob, and PP females. Data are represented as mean ± SEM. One-way-ANOVA and Tukey's test were used. F(2, 10) = 7.959, ∗p < 0.01. .