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. 2020 Sep 28;10:15922. doi: 10.1038/s41598-020-73074-4

Figure 2.

Figure 2

The effect of TPA treatment on the number of derived cardiomyocytes—indirect quantification. The number of cardiomyocytes was determined in HG8 cells treated with 0.1% DMSO and 1 µM TPA in different time windows (see Fig. 1). The selection of cardiomyocytes began on Day 14 and measurements were performed on Day 20 of differentiation (A, B). Selection of cardiomyocytes began on Day 20 and measurements were performed on Day 26 of differentiation (C, D). Data are presented as mean ± SEM, n ≥ 4. Statistical significance was determined by ANOVA with post hoc Bonferroni's Multiple Comparison test; *P < 0.05; **P < 0.01; ***P < 0.001.