Fig. 1. DDB2 and XPC are differently regulated by downstream factors.
a Fluorescence Recovery After Photobleaching (FRAP) analysis of DDB2 mobility in mock or UV-C irradiated (10 J/m2) VH10 cells stably expressing GFP-DDB2 and transfected with control (CTRL) or GTF2H1 siRNAs. GFP-DDB2 fluorescence recovery was measured in a strip across the nucleus after bleaching, normalized to bleach depth, and the average pre-bleach intensities (1.0). b Percentage of GFP-DDB2 immobile fraction in VH10 fibroblasts treated with control (CTRL), GTF2H1 or XPG siRNAs, determined from FRAP analyses as depicted in (a). Percentage immobile fraction represents the ratio between the average recovered fluorescence intensity of UV- and mock-treated cells, over the last 10 s of the measurements, as explained in the methods. c FRAP analysis of XPC mobility in mock or UV-C irradiated (10 J/m2) XP4PA cells stably expressing XPC-GFP and transfected with control (CTRL) or GTF2H1 siRNAs. XPC-GFP-fluorescence recovery was measured and normalized as described in (a). d Percentage of XPC-GFP immobile fraction in XP4PA cells treated with control (CTRL), GTF2H1 or XPG siRNAs, determined by FRAP analysis as depicted in (c) and described in (b). Graphs and FRAP curves depict the mean & S.E.M. of >30 cells from three independent experiments. **P < 0.01, ***P < 0.001, relative to siCTRL control 10 J/m2, analyzed by unpaired, two-tailed t-test (adjusted for multiple comparisons, see “Methods”). Source data are provided as a Source Data file.