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. 2020 Sep 28;10:15915. doi: 10.1038/s41598-020-72952-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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3D cell culturing wells in the PDMS flow chips. The concave shaped wells in the PDMS flow chips were designed to assist in the aggregation of cells following the deposit of HT29 cells in suspension. On Day 0 cells would be added to the wells forming cell aggregates which would then be cultured under static conditions for another 2 days to form 3D spheroids. Fluid flow would then be applied through the wells following the formation of 3D spheroids.