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. 2020 Sep 28;10:15934. doi: 10.1038/s41598-020-72942-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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R205A attenuates anchorage-independent growth, migration and proliferation of CRC cells. (A) Anchorage-independent growth assays performed in CRC cell lines with vector Ctrl, WT-YBX1 or mutant R205A. Right panel shows quantified data. (B) Boyden Chamber cell migration assays performed with same cells as above. Right panel shows quantified data. (C) Cell growth assay performed in a panel of CRC cell lines expressing vector Ctrl, WT-YBX1 or mutant R205A. Cell number was assessed at different days over a time course of 9 days. The data represent the means ± SEM or S.D. for at least three independent experiments. *p < 0.05 vs. Ctrl. ^#p < 0.05 vs. WT-YBX1.