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IL-1β and TNF-α promote NF-κB p65 translocation to the nucleus of HER2-negative DCIS cells. MCF10A and MCF10A(HER2+) cells were serum starved overnight and treated with A) IL-1β (10 ng/ml) or B) TNF-α (10 ng/ml) for 30 min. NF-κB p65 translocation to the nucleus was assessed with fluorescent microscopy, scale bar represents 20 µm.
