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. 2020 Sep 28;11:4878. doi: 10.1038/s41467-020-18702-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Tumor mutation burden (TMB) of pulmonary sarcomatoid carcinoma (PSC) and The Cancer Genome Atlas (TCGA) tumor types, ordered by median. Somatic single-nucleotide variants and small indels of PSC were called using MuTect (version 3.1-0-g72492bb) and Strelka (version 1.0.14) via an in-house computational pipeline, and the mutation list of TCGA cancer types was released by the Pan-Cancer Atlas consortium (https://gdc.cancer.gov/about-data/publications/pancanatlas, mc3.v0.2.8.PUBLIC.maf.gz). b Leukocyte fraction (LF) of PSC and TCGA tumor types, ordered by median. The LF of all TCGA samples was collected from a previous study, and we estimated the LF of PSC using the algorithm provided by the same study⁵⁸. Center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range; points, outliers. c Spearman correlation of the fluorescence intensity of CD4, CD8, and CD68 between epithelial and sarcomatoid components. Source data are provided as a Source data file. d Representative images of fluorescent multiplex immunohistochemical analysis of P46 and P48. E and S represent epithelial and sarcomatoid components, respectively. Scale bar: 50 μm. The fluorescent multiplex immunohistochemical analysis was performed on the epithelial and sarcomatoid components of 14 patients. CD4, green; CD8, cyan; CD68, red; PD-L1, orange; FOXP3, magenta. CD4, cluster of differentiation 4; CD8, cluster of differentiation 8; CD68, cluster of differentiation 68.